Agrin drives synaptogenesis

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Agrin isoforms and their role in synaptogenesis.

Agrin is thought to mediate the motor neuron-induced aggregation of synaptic proteins on the surface of muscle fibers at neuromuscular junctions. Recent experiments provide direct evidence in support of this hypothesis, reveal the nature of agrin immunoreactivity at sites other than neuromuscular junctions, and have resulted in findings that are consistent with the possibility that agrin plays ...

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During neuromuscular synapse formation, motor axons induce clustering of acetylcholine receptors (AChRs) in the muscle fiber membrane. The protein agrin, originally isolated from the basal lamina of the synaptic cleft, is synthesized and secreted by motoneurons and triggers formation of AChR clusters on cultured myotubes. We show here postsynaptic AChR aggregates are markedly reduced in number,...

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Agrin mediates a rapid switch from electrical coupling to chemical neurotransmission during synaptogenesis

In contrast to its well-established actions as an organizer of synaptic differentiation at the neuromuscular junction, the proteoglycan agrin is still in search of a function in the nervous system. Here, we report an entirely unanticipated role for agrin in the dual modulation of electrical and chemical intercellular communication that occurs during the critical period of synapse formation. Whe...

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Neuritic deposition of agrin on culture substrate: implications for nerve-muscle synaptogenesis.

Recent experiments have indicated that neural agrin is deposited at newly forming nerve-muscle synapses and has a primary synaptogenic role there. As a step toward assessing how the spatial arrangement of new synaptic sites is regulated, we compared the pattern of agrin deposition by Xenopus neurites on culture substrate and on muscle cells. The neurons were grown on a substrate that bound thei...

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Synaptogenesis

Synapses are specialized asymmetric cell-cell connections permitting the controlled transfer of an electrical or chemical signal between a presynaptic neuronal cell and a postsynaptic target cell (e.g. neuron or muscle). Adequate synapse function is an essential prerequisite of all neuronal processing, including higher cognitive functions, such as learning and memory. At synapses, neurotransmit...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 2005

ISSN: 1540-8140,0021-9525

DOI: 10.1083/jcb1693iti2